Identification of the cystic fibrosis gene: chromosome walking and jumping.

@article{Rommens1989IdentificationOT,
  title={Identification of the cystic fibrosis gene: chromosome walking and jumping.},
  author={Johanna M Rommens and Michael C. Iannuzzi and Bat-sheva Kerem and Mitchell L. Drumm and Georg Melmer and M. Dean and Richard F. Rozmahel and Joe L Cole and Derek Kennedy and Nami Hidaka},
  journal={Science},
  year={1989},
  volume={245 4922},
  pages={1059-65}
}
An understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several… CONTINUE READING
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