Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.

@article{Riordan1989IdentificationOT,
  title={Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.},
  author={J. Riordan and J. Rommens and B. Kerem and N. Alon and R. Rozmahel and Z. Grzelczak and J. Zieleński and S. Lok and N. Plavsic and J. Chou},
  journal={Science},
  year={1989},
  volume={245 4922},
  pages={
          1066-73
        }
}
Key MethodOverlapping complementary DNA clones were isolated from epithelial cell libraries with a genomic DNA segment containing a portion of the putative cystic fibrosis (CF) locus, which is on chromosome 7. Transcripts, approximately 6500 nucleotides in size, were detectable in the tissues affected in patients with CF. The predicted protein consists of two similar motifs, each with (i) a domain having properties consistent with membrane association and (ii) a domain believed to be involved in ATP…Expand
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