Identification of the cloned S. cerevisiae LYS2 gene by an integrative transformation approach

@article{Eibel1983IdentificationOT,
  title={Identification of the cloned S. cerevisiae LYS2 gene by an integrative transformation approach},
  author={Hermann Eibel and Peter Philippsen},
  journal={Molecular and General Genetics MGG},
  year={1983},
  volume={191},
  pages={66-73}
}
We isolated the LYS2 gene of S. cerevisiae on an autonomously replicating plasmid in a four-step procedure. First, we identified a recombinant plasmid which expressed a lys2 complementing activity upon yeast transformation of a lys2 mutant. Second, we determined the boundaries of the corresponding transcribed sequence in this plasmid by S1 nuclease mapping of the mRNA. Third, we inactivated the functional chromosomal copy coding for the lys2 complementing activity by directed integration of a… CONTINUE READING

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