Identification of the catalytic residue of Thermococcus litoralis 4-alpha-glucanotransferase through mechanism-based labeling.

@article{Imamura2001IdentificationOT,
  title={Identification of the catalytic residue of Thermococcus litoralis 4-alpha-glucanotransferase through mechanism-based labeling.},
  author={Hiromi Imamura and Shinya Fushinobu and Byoung Soo Jeon and Takayoshi Wakagi and Hiroshi Matsuzawa},
  journal={Biochemistry},
  year={2001},
  volume={40 41},
  pages={
          12400-6
        }
}
Thermococcus litoralis 4-alpha-glucanotransferase (TLGT) belongs to family 57 of glycoside hydrolases and catalyzes the disproportionation and cycloamylose synthesis reactions. Family 57 glycoside hydrolases have not been well investigated, and even the catalytic mechanism involving the active site residues has not been studied. Using 3-ketobutylidene-beta-2-chloro-4-nitrophenyl maltopentaoside (3KBG5CNP) as a donor and glucose as an acceptor, we showed that the disproportionation reaction of… CONTINUE READING
Highly Cited
This paper has 22 citations. REVIEW CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 12 extracted citations