Identification of the canarypox virus thymidine kinase gene and insertion of foreign genes.

@article{Amano1999IdentificationOT,
  title={Identification of the canarypox virus thymidine kinase gene and insertion of foreign genes.},
  author={Hidenori Amano and S. R. K. Morikawa and Hidetada Shimizu and Ikuo Shoji and D Kurosawa and Yoshiharu Matsuura and Tatsuo Miyamura and Yoshiaki Ueda},
  journal={Virology},
  year={1999},
  volume={256 2},
  pages={280-90}
}
We mapped the canarypox virus (CaPV) thymidine kinase (TK) gene within a 5.8-kbp XbaI fragment of the genome by Southern blotting using the fowlpox virus (FPV) TK gene as a probe. Nucleotide sequence analysis of the fragment revealed seven open reading frames (ORFs) showing gene organization similar to that of FPV. The TK gene contained in this region had an ORF of 179 amino acids encoding a polypeptide with a putative molecular mass of 20.0 kDa. An A/T-rich region and a transcription… CONTINUE READING