Identification of the blood-borne somatotroph-differentiating factor during chicken embryonic development.

@article{Morpurgo1997IdentificationOT,
  title={Identification of the blood-borne somatotroph-differentiating factor during chicken embryonic development.},
  author={Benjamin Morpurgo and Carlton E. Dean and Tom E. Porter},
  journal={Endocrinology},
  year={1997},
  volume={138 11},
  pages={4530-5}
}
Somatotrophs become a significant population by day 16 of chicken embryonic development. We have previously demonstrated that an earlier induction of GH cell differentiation is possible with the addition of day 16 embryonic serum to cultures of day 12 pituitary cells, an age when somatotrophs are rare. The present study was designed to identify the blood-borne signal(s) responsible for the serum activity, using reverse hemolytic plaque assays to identify individual GH-secreting cells. The… CONTINUE READING