Identification of the amino acid residues responsible for the reversible photoconversion of the monomeric red fluorescent protein TagRFP

Abstract

The site-directed mutagenesis of the monomeric red fluorescent protein TagRFP and its variants was performed with the goal of generating reversibly photoactivatable fluorescent proteins. Amino acids at positions 69, 148, 165, 179, and 181 (enumeration according to the green fluorescent protein GFP) were shown to play a key role in the manifestation of the… (More)
DOI: 10.1134/S1068162010020068

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