Identification of the active site nucleophile in jack bean alpha-mannosidase using 5-fluoro-beta-L-gulosyl fluoride.

@article{Howard1998IdentificationOT,
  title={Identification of the active site nucleophile in jack bean alpha-mannosidase using 5-fluoro-beta-L-gulosyl fluoride.},
  author={Shavonne Howard and Shouming He and Stephen G Withers},
  journal={The Journal of biological chemistry},
  year={1998},
  volume={273 4},
  pages={2067-72}
}
Mannosidases play a key role in the processing of glycoproteins and thus are of considerable pharmaceutical interest and indeed have emerged as targets for the development of anti-cancer therapies. Access to useful quantities of the mammalian enzymes has not yet been achieved; therefore, jack bean mannosidase, a readily available enzyme, has become the… CONTINUE READING