Identification of the P 2 Y 12 Receptor in Nucleotide Inhibition of Exocytosis from Bovine Chromaffin Cells

@inproceedings{Ennion2004IdentificationOT,
  title={Identification of the P 2 Y 12 Receptor in Nucleotide Inhibition of Exocytosis from Bovine Chromaffin Cells},
  author={Steven J. Ennion and Andrew Powell and Elizabeth P. Seward},
  year={2004}
}
Nucleotides are released from bovine chromaffin cells and take part in a feedback loop to inhibit further exocytosis. To identify the nucleotide receptors involved, we measured the effects of a range of exogenous nucleotides and related antagonists on voltage-operated calcium currents (ICa), intracellular calcium concentration ([Ca ]i), and membrane capacitance changes. In comparative parallel studies, we also cloned the bovine P2Y12 receptor from chromaffin cells and determined its properties… CONTINUE READING