Identification of the Mechanisms Causing Reversion to Virulence in an Attenuated SARS-CoV for the Design of a Genetically Stable Vaccine

@inproceedings{JimnezGuardeo2015IdentificationOT,
  title={Identification of the Mechanisms Causing Reversion to Virulence in an Attenuated SARS-CoV for the Design of a Genetically Stable Vaccine},
  author={Jose M. Jim{\'e}nez-Guarde{\~n}o and Jose A. Regla-Nava and Jose L. Nieto-Torres and Marta L. DeDiego and Carlos Casta{\~n}o-Rodr{\'i}guez and Ra{\'u}l Fern{\'a}ndez-Delgado and Stanley Perlman and Luis Enjuanes and Christian Drosten},
  booktitle={PLoS pathogens},
  year={2015}
}
A SARS-CoV lacking the full-length E gene (SARS-CoV-∆E) was attenuated and an effective vaccine. Here, we show that this mutant virus regained fitness after serial passages in cell culture or in vivo, resulting in the partial duplication of the membrane gene or in the insertion of a new sequence in gene 8a, respectively. The chimeric proteins generated in… CONTINUE READING