Identification of the Escherichia coli sohB gene, a multicopy suppressor of the HtrA (DegP) null phenotype.

@article{Baird1991IdentificationOT,
  title={Identification of the Escherichia coli sohB gene, a multicopy suppressor of the HtrA (DegP) null phenotype.},
  author={Lisa Baird and Barbara Lipińska and Surabhi Raina and CJ. Georgopoulos},
  journal={Journal of bacteriology},
  year={1991},
  volume={173 18},
  pages={5763-70}
}
We cloned and sequenced the sohB gene of Escherichia coli. The temperature-sensitive phenotype of bacteria that carry a Tn10 insertion in the htrA (degP) gene is relieved when the sohB gene is present in the cell on a multicopy plasmid (30 to 50 copies per cell). The htrA gene encodes a periplasmic protease required for bacterial viability only at high temperature, i.e., above 39 degrees C. The sohB gene maps to 28 min on the E. coli chromosome, precisely between the topA and btuR genes. The… CONTINUE READING

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