Identification of the Components Controlling Inactivation of Voltage-Gated Ca2+ Channels

  title={Identification of the Components Controlling Inactivation of Voltage-Gated Ca2+ Channels},
  author={James K Kim and Smita Ghosh and Deborah A. Nunziato and Geoffrey S Pitt},
Ca(2+)-dependent inactivation (CDI) of L-type voltage-gated Ca(2+) channels limits Ca(2+) entry into neurons, thereby regulating numerous cellular events. Here we present the isolation and purification of the Ca(2+)-sensor complex, consisting of calmodulin (CaM) and part of the channel's pore-forming alpha(1C) subunit, and demonstrate the Ca(2+)-dependent conformational shift that underlies inactivation. Dominant-negative CaM mutants that prevent CDI block the sensor's Ca(2+)-dependent… CONTINUE READING

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