Identification of the Beet Cyst Nematode Heterodera schachtii by PCR

@article{Amiri2004IdentificationOT,
  title={Identification of the Beet Cyst Nematode Heterodera schachtii by PCR},
  author={S. Amiri and S. Subbotin and M. Moens},
  journal={European Journal of Plant Pathology},
  year={2004},
  volume={108},
  pages={497-506}
}
PCR-RFLPs of ITS-rDNA and PCR with species-specific primers were developed for identification of cysts and juveniles of the beet cyst nematode Heterodera schachtii. [...] Key Result Restrictions of PCR product by MvaI or ScrFI distinguish H. schachtii, H. betae, H. trifolii and H. medicaginis. RFLP profiles with eight restriction enzymes for these four nematode species are presented.Expand

Figures and Tables from this paper

Isolation and characterization of microsatellite loci in the sugar beet cyst nematode Heterodera schachtii
TLDR
The isolation of five microsatellites loci from the sugar beet cyst nematode (Heterodera schachtii) is reported, which will be used to study the genetic structure of this obligatory parasite and how it is affected by the use of resistant plants. Expand
Development of a species-specific PCR to detect the cereal cyst nematode, Heterodera latipons
TLDR
Several Heterodera species can reduce the yield of wheat and barley, among which H. avenae, H. filipjevi and H. latipons are economically the most important, and species-specific primers for the detection are developed. Expand
Species-Specific PCR Assays for Differentiating Heterodera filipjevi and H. avenae.
TLDR
This is the first report of a species-specific ITS PCR assay to detect and identify H. filipjevi and H. avenae, which will enhance diagnosis of cereal cyst nematode species in infested fields. Expand
Identification of Heterodera glycines (Tylenchida; Heteroderidae) Using qPCR
TLDR
The findings suggested that H. glycines could be distinguished from H. sojae and other Heterodera species when a qPCR assay is used with a specific primer set, which is crucial for preventing crop damage and in decision making for controlling this nematode. Expand
Identification of Pratylenchus thornei, the cereal and legume root-lesion nematode, based on SCAR-PCR and satellite DNA
TLDR
The described SCAR-PCR-based assay has the potential to be optimized for routine practical diagnostic tests and the use of a species-specific satellite DNA sequence to distinguish P. thornei from other Pratylenchus spp. Expand
Quantitative detection of the potato cyst nematode, Globodera pallida, and the beet cyst nematode, Heterodera schachtii, using Real-Time PCR with SYBR green I dye.
TLDR
The Real-Time PCR assay with SYBR green I dye targeting fragments of the ITS-rDNA provided a sensitive means for the rapid and simultaneous detection and quantification of juveniles of these pests. Expand
Molecular identification of Heterodera spp., an overview of fifteen years of research.
TLDR
Researchers distinguish most of the agrieulturally important eyst nematode species from one another and from their sibling species by restricting the lTS amplicons with one or a combination of seven restrietion enzymes, but more eonclusive moleeular identification tools are needed to diagnose some species. Expand
Characterization of 15 microsatellite loci and genetic analysis of Heterodera schachtii (Nematoda: Heteroderidae) in South Korea.
TLDR
Genetic diversity analysis suggested that the current infestation in South Korea may have come either from a single source population of mixed ancestry, or from multiple sources, indicating that implementing adequate prevention measures is still an unmet challenge. Expand
Development of two species-specific primer sets to detect the cereal cyst nematodes Heterodera avenae and Heterodera filipjevi
TLDR
Two sets of species-specific primers were identified for the identification of both species and the conditions for their use in PCR were optimised, allowing the detection of H. avenae and H. filipjevi where they occur in mixed populations with other Heterodera spp. Expand
Integrative diagnosis of carrot cyst nematode (Heterodera carotae) using morphology and several molecular markers for an accurate identification
TLDR
Cyst nematodes obtained from commercial carrot fields in Ontario (Canada) and northern and southern Italy were subjected to morphological and molecular examination and a diagnostic conventional PCR method was developed based on a primer set to be specific for H. carotae using coxI sequence. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 41 REFERENCES
Identification of Cyst Nematodes of Agronomic and Regulatory Concern with PCR-RFLP of ITS1.
TLDR
It was demonstrated that ITS1 heterogeneity existed within individuals and between isolates, but did not result in different restriction patterns, as previously reported. Expand
Differentiation of Australasian potato cyst nematode (PCN) populations using the polymerase chain reaction (PCR)
TLDR
Molecular examination of the ribosomal internal transcribed spacer (ITS) region in potato cyst nematodes (PCN) is described and Polymerase chain reaction (PCR) primers based upon the G. rostochiensis–G. Expand
A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR
TLDR
A method for rapid identification of juveniles and cysts of the soybean cyst nematode based on PCR with species specific primers is described and a single cyst or second stage juvenile of Heterodera glycines alone or in a mixture with other soil inhabiting nematodes was detectable. Expand
The rDNA Internal Transcribed Spacer Region as a Taxonomic Marker for Nematodes.
TLDR
IT heterogeneity in individuals and populations was identified in several nematode taxa and PCR-RFLP of ITS1 is advocated as a method of taxonomic analysis in genera such as Helicotylenchus that contain numerous species with few diagnostic morphological characteristics. Expand
Identification of Pratylenchus Coffeae and P. Loosi Using Specific Primers for PCR Amplification of Ribosomal DNA
The 18-26S region of rDNA of P. coffeae and P. loosi were amplified by PCR from genomic DNA. Sequence analyses indicated that the 5.8S gene sequences were conserved whereas the internal transcribedExpand
A PCR Assay to Identify and Distinguish Single Juveniles of Meloidogyne hapla and M. chitwoodi.
Random amplified polymorphic DNA (RAPD) bands that distinguish Meloidogyne hapla and M. chitwoodi from each other, and from other root-knot nematode species, were identified using a series of randomExpand
The host range of the sugar beet nematode, Heterodera schachtii Schmidt
The life cycle of the sugar beet nematode is essentially typical of all known species of the genus H eterodem. In the presence of growing host plants, eggs ha tch and la rvae escape from pro­ tectiveExpand
Distribution and economic importance
TLDR
The family Heteroderidae of plant parasitic nematodes contains the cyst-forming species within the sub-family Heterodinae, within which the numbers of genera and species described have increased greatly over the last five decades. Expand
Potential for identification of quarantine nematodes by PCR1
TLDR
It is suggested that international cooperation is needed in order to build up banks of DNA restriction patterns characteristic of each quarantine nematode species. Expand
Differences between ITS regions of isolates of the root-knot nematodes Meloidogyne hapla and M. chitwoodi
TLDR
Results indicated that different ITS sequences are present within a single individual of M. chitwoodi proper, and these sequences could be distinguished by ITS restriction fragment length polymorphisms. Expand
...
1
2
3
4
5
...