Identification of the Arabidopsis thaliana Flavonoid 3'-Hydroxylase Gene and Functional Expression of the Encoded P450 Enzyme

@inproceedings{Schoenbohm2000IdentificationOT,
  title={Identification of the Arabidopsis thaliana Flavonoid 3'-Hydroxylase Gene and Functional Expression of the Encoded P450 Enzyme},
  author={C Schoenbohm and Stefan Martens and Christiane Eder and Gert Forkmann and Bernd Weisshaar},
  booktitle={Biological chemistry},
  year={2000}
}
Abstract The phenylpropanoid pathway results in the synthesis of thousands of compounds, including flavonoids like flavonols, anthocyanidins and tannins. In Arabidopsis thaliana, the lack of tannins in the seed coat (testa) causes the transparent testa (tt) phenotype. In the present study, we identified the gene responsible for the tt7 mutation. We show that TT7 encodes the enzyme flavonoid 3′-hydroxylase (F3′H), and demonstrate that this P450-dependent monooxygenase has F3′H activity. The… 
View on PubMed
floramare.de
190 Citations
Highly Influential Citations
12
Background Citations
79
Methods Citations
12
Results Citations
2

Figures from this paper

190 Citations

Citation Type

Citation Type

Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase
TLDR
Evidence that the remaining flavonol glycosides found in the fls1-2 mutant are synthesized in planta by the FLS-like side activity of the LDOX enzyme is presented.
The flavonoid biosynthetic pathway in Arabidopsis: structural and genetic diversity.
Molecular and biochemical characterization of torenia flavonoid 3′-hydroxylase and flavone synthase II and modification of flower color by modulating the expression of these genes
Functional conservation of plant secondary metabolic enzymes revealed by complementation of Arabidopsis flavonoid mutants with maize genes.
TLDR
It is concluded that enzymes involved in secondary metabolism can be functionally exchangeable between plants separated by large evolutionary distances, in sharp contrast to the notion that the more relaxed selective constrains to which secondary metabolic pathways are subjected is responsible for the rapid divergence of the corresponding enzymes.
Biochemical and genetic characterization of Arabidopsis flavanone 3beta-hydroxylase.
Expression of a putative flavonoid 3′-hydroxylase in sorghum mesocotyls synthesizing 3-deoxyanthocyanidin phytoalexins
The b Gene of Pea Encodes a Defective Flavonoid 3′,5′-Hydroxylase, and Confers Pink Flower Color1[W][OA]
TLDR
The molecular characterization of genes involved in pigmentation in pea provides valuable anchor markers for comparative legume genomics and will help to identify differences in anthocyanin biosynthesis that lead to variation in Pigmentation among legume species.
In vitro properties of a recombinant flavonol synthase from Arabidopsis thaliana.
Molecular cloning and functional characterization of the flavonoid 3 ́-hydroxylase gene from Rubus coreanus
TLDR
The cloning and molecular characterization of RcMF3′H1 will facilitate a better insight into the anthocyanin biosynthesis pathway in R. coreanus Miquel.
Cloning and Characterization of a Flavonoid 3′-Hydroxylase Gene from Tea Plant (Camellia sinensis)
TLDR
In the study of nitrogen supply for the tea plant growth, the results showed the expression level of CsF3′H and all other tested genes increased in response to nitrogen depletion after 12 days of treatment, in agreement with a corresponding increase in 3′,4′-catechins, 3′-4′,5′- catechins and flavan 3-ols content in the leaves.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 28 REFERENCES
Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis.
TLDR
Tissue- and locus-specific differences in the flavonols and anthocyanidins synthesized by mutant and wild-type plants are identified and evidence was uncovered that tt8 and ttg specifically affect dihydroflavonol reductase gene expression.
The TRANSPARENT TESTA GLABRA1 Locus, Which Regulates Trichome Differentiation and Anthocyanin Biosynthesis in Arabidopsis, Encodes a WD40 Repeat Protein
The TRANSPARENT TESTA GLABRA1 (TTG1) locus regulates several developmental and biochemical pathways in Arabidopsis, including the formation of hairs on leaves, stems, and roots, and the production of…
Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida.
We have isolated a cDNA clone that corresponds to the Ht1 locus of petunia which controls the hydroxylation of dihydrokaempferol to dihydroquercetin and of naringenin to eriodictyol by the action of…
Characterization of tt15, a novel transparent testa mutant of Arabidopsis thaliana (L.) Heynh.
TLDR
Analysis of pigment accumulation and the study of expression patterns of genes involved in flavonoid biosynthesis in tt15 plants and seeds indicated a seed-specific phenotype, which was consistent with the maternal origin of the testa.
Characterization of recombinant plant cinnamate 4-hydroxylase produced in yeast. Kinetic and spectral properties of the major plant P450 of the phenylpropanoid pathway.
TLDR
Results indicate that the microsomal system constituted by the overexpressed yeast P450 reductase and CA4H is characterized by a 1:1 coupling between NADPH oxidation and cinnamate hydroxylation and by one of the highest turnover numbers reported for an NADPH-dependent P450 reaction.
Molecular cloning, sequence analysis, and in vitro expression of flavanone 3 beta-hydroxylase from Petunia hybrida.
Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes.
TLDR
These experiments demonstrate the efficiency of the screening method and gene disruption strategy used for assigning functions to genes defined only by sequence and show that TRANSPARENT TESTA 6 (TT6) encodes flavanone 3-hydroxylase.
Genetics and Biochemistry of Anthocyanin Biosynthesis.
TLDR
The characterization of genetically defined mutations has enabled the order of many reactions in anthocyanin synthesis and their modification to be elucidated, and the more recent developments in gene isolation and characterization are concentrated.
Conversion of Flavanone to Flavone, Dihydroflavonol and Flavonol with an Enzyme System from Cell Cultures of Parsley
TLDR
It is postulate that synthesis of flavone and flavonol from flavanone proceeds via 2-hydroxy- and 2,3-dihydroxyflavanone, respectively, with subsequent dehydration, and in vitro biosynthesis of radioactive flavone, dihydroflavonol and flav onol from [2-14C]malonyl-CoA and 4-coumaroyl- CoA in good yield and with high specific activity is possible.
Plant Cytochrome P450 Monooxygenases
TLDR
Biochemical and molecular characterizations on a number of plant P450s have indicated that the relationships between these heme proteins and their substrates are at least as complex as previously thought.
...
1
2
3
...