Identification of the 16S rRNA m5C967 methyltransferase from Escherichia coli.

  title={Identification of the 16S rRNA m5C967 methyltransferase from Escherichia coli.},
  author={Xiang-rong Gu and Claes Gustafsson and Jakang Ku and Minmin Yu and Daniel V Santi},
  volume={38 13},
The fmu gene product has been proposed to be an RNA methyltransferase [Koonin, E. V. (1994) Nucleic Acids Res. 22, 2476-2478]. Fmu has been cloned and expressed, and the encoded 47 kDa protein has been purified and characterized. The enzyme catalyzed specific methylation of C967 of unmodified 16S rRNA transcripts. A 16mer stem-loop structure containing C967 (nt 960-975) was also a good substrate for the enzyme in vitro. Methylation of C967 was confirmed by several methods including analysis of… CONTINUE READING
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