Identification of suitable reference genes for studying gene expression in cucumber plants subjected to abiotic stress and growth regulators

@article{Migocka2010IdentificationOS,
  title={Identification of suitable reference genes for studying gene expression in cucumber plants subjected to abiotic stress and growth regulators},
  author={Magdalena Migocka and Anna Papierniak},
  journal={Molecular Breeding},
  year={2010},
  volume={28},
  pages={343-357}
}
It has been shown that genes considered to be valid reference genes using semi-quantitative techniques (e.g. northern blot) appear to be less reliable when highly sensitive real-time PCR (qPCR) or microarrays are used. Therefore, the validation of expression stability of reference genes has become an important component of any study using such types of assay. No reference genes have been validated for expression studies of cucumber genes to date. Since the genome of this widely cultivated crop… CONTINUE READING

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