Identification of specific protein carbonylation sites in model oxidations of human serum albumin.

@article{Temple2006IdentificationOS,
  title={Identification of specific protein carbonylation sites in model oxidations of human serum albumin.},
  author={Ani Temple and Ten-Yang Yen and Scott Gronert},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2006},
  volume={17 8},
  pages={1172-80}
}
Human serum albumin (HSA) was subjected to oxidative stress and the locations of the resulting protein carbonyls were determined using mass spectrometry in conjunction with a hydrazide labeling scheme. To model oxidative stress, HSA samples were subjected to metal-catalyzed oxidation (MCO) conditions or treated with hypochlorous acid (HOCl). Oxidation led to the conversion of lysine residues to 2-aminoadipic semi-aldehyde residues, which were subsequently labeled with biotin hydrazide. Analysis… CONTINUE READING
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