Identification of soluble protein fragments by gene fragmentation and genetic selection

@inproceedings{Dyson2008IdentificationOS,
  title={Identification of soluble protein fragments by gene fragmentation and genetic selection},
  author={Michael R Dyson and Rajika L Perera and S Paul Shadbolt and Lynn Biderman and Krystyna Bromek and Natalia V. Murzina and John McCafferty},
  booktitle={Nucleic acids research},
  year={2008}
}
We describe a new method, which identifies protein fragments for soluble expression in Escherichia coli from a randomly fragmented gene library. Inhibition of E. coli dihydrofolate reductase (DHFR) by trimethoprim (TMP) prevents growth, but this can be relieved by murine DHFR (mDHFR). Bacterial strains expressing mDHFR fusions with the soluble proteins green fluroscent protein (GFP) or EphB2 (SAM domain) displayed markedly increased growth rates with TMP compared to strains expressing insoluble… CONTINUE READING

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