Identification of sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides) by PCR amplification of the 5S rDNA gene.

@article{Cspedes1999IdentificationOS,
  title={Identification of sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides) by PCR amplification of the 5S rDNA gene.},
  author={{\'A}ngel Enrique C{\'e}spedes and Teresa Garcia and Erkuden P{\'e}rez Carrera and Isabel Gonz{\'a}lez and Alicia Calleja Fern{\'a}ndez and Pablo V. Hernandez and Rosario Mart{\'i}n},
  journal={Journal of agricultural and food chemistry},
  year={1999},
  volume={47 3},
  pages={1046-50}
}
Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene, has been used for the identification of sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides). Two species-specific primers were designed to amplify specific fragments of the 5S rDNA gene in each species. The remarkably different size of the amplicons obtained gives, by simple agarose gel electrophoresis, two distinguishable band patterns for both flatfish species. This genetic marker can be very useful… CONTINUE READING

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