Identification of side population cells in human hepatocellular carcinoma cell lines with stepwise metastatic potentials

@article{Shi2008IdentificationOS,
  title={Identification of side population cells in human hepatocellular carcinoma cell lines with stepwise metastatic potentials},
  author={Guo-Ming Shi and Yang Xu and Jia Fan and Jian Zhou and Xin-Rong Yang and Shuang-jian Qiu and Yong Liao and Wei-zhong Wu and Yuan Ji and Ai-Wu Ke and Zhen-bin Ding and Yi-zhou He and B Wu and Guo-huan Yang and Wen-zhen Qin and Wu Zhang and Jiang Yi Zhu and Zhi-Hui Min and Zhi-quan Wu},
  journal={Journal of Cancer Research and Clinical Oncology},
  year={2008},
  volume={134},
  pages={1155-1163}
}
To identify the side population (SP) cells from four hepatocellular carcinoma (HCC) cell lines with stepwise metastatic potentials. SP cells were sorted from HCCLM3, MHCC97-H, MHCC97-L and Hep3B by flow cytometry, and then analyzed by differentiation study, clonogenic assay, chemoresistance study and tumorigenicity assay in vivo. The expression of ABCG2 in SP cells was detected by immunocytochemistry, western blotting and real-time quantitative PCR, respectively. There was significant… CONTINUE READING