Identification of sex chromosome molecular markers using RAPDs and fluorescent in situ hybridization in rainbow trout

@article{Iturra1997IdentificationOS,
  title={Identification of sex chromosome molecular markers using RAPDs and fluorescent in situ hybridization in rainbow trout},
  author={Patricia Iturra and Juan F Medrano and Mark Bagley and Natalia Lam and Nora Vergara and Juan Carlos Mar{\'i}n},
  journal={Genetica},
  year={1997},
  volume={101},
  pages={209-213}
}
The goal of this work is to identify molecular markers associated with the sex chromosomes in rainbow trout to study the mode of sex determination mechanisms in this species. Using the RAPD assay and bulked segregant analysis, two markers were identified that generated polymorphic bands amplifying preferentially in males of the Mount Lassen and Scottish strains of rainbow trout. Chromosomal localization using fluorescent in situ hybridization of a 900 bp probe developed from one of these… CONTINUE READING
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