Identification of seven hydrophobic clusters in GCN4 making redundant contributions to transcriptional activation.

@article{Jackson1996IdentificationOS,
  title={Identification of seven hydrophobic clusters in GCN4 making redundant contributions to transcriptional activation.},
  author={Belinda M. Jackson and C M Drysdale and Krishnamurthy Natarajan and Alan G Hinnebusch},
  journal={Molecular and cellular biology},
  year={1996},
  volume={16 10},
  pages={5557-71}
}
GCN4 is a transcriptional activator in the bZIP family that regulates amino acid biosynthetic genes in the yeast Saccharomyces cerevisiae. The N-terminal 100 amino acids of GCN4 contains a potent activation function that confers high-level transcription in the absence of the centrally located acidic activation domain (CAAD) delineated in previous studies. To identify specific amino acids important for activation by the N-terminal domain, we mutagenized a GCN4 allele lacking the CAAD and… CONTINUE READING