Identification of selenomethionine in selenized yeast using two-dimensional liquid chromatography-mass spectrometry based proteomic analysis.

@article{McSheehy2005IdentificationOS,
  title={Identification of selenomethionine in selenized yeast using two-dimensional liquid chromatography-mass spectrometry based proteomic analysis.},
  author={Shona McSheehy and John Arthur Kelly and Luc Tessier and Zolt{\'a}n Mester},
  journal={The Analyst},
  year={2005},
  volume={130 1},
  pages={
          35-7
        }
}
Selenium-enriched yeast has been commonly used as a nutritional supplement. Here we describe a protocol used to investigate the metabolic fate of inorganic selenium in yeast. We provide definitive, mass spectrometry based evidence for the non-specific incorporation of selenomethionine in the yeast proteome involving the replacement of about 30% of all methionine with selenomethionine. 

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