Identification of selenium species in urine by ion-pairing HPLC–ICP–MS using laboratory-synthesized standards

@article{Wrobel2003IdentificationOS,
  title={Identification of selenium species in urine by ion-pairing HPLC–ICP–MS using laboratory-synthesized standards},
  author={Kazimierz Wrobel and Katarzyna Wrobel and Sasi S. Kannamkumarath and Joseph A. Caruso},
  journal={Analytical and Bioanalytical Chemistry},
  year={2003},
  volume={377},
  pages={670-674}
}
This study focused on the detection/identification of possible selenium metabolites in human urine. Organoselenium compounds not commercially unavailable were synthesized and characterized by electrospray mass spectrometry. Separation of selenomethionine, methylselenomethionine, trimethylselonium, selenoethionine, and selenoadenosylmethionine was achieved by ion-pairing HPLC with a mobile phase of 2 mmol L−1 hexanesulfonic acid, 0.4% acetic acid, 0.2% triethanolamine (pH 2.5), and 5% methanol… CONTINUE READING