Identification of sample-specific sequences in mammalian cDNA and genomic DNA by the novel ligation-mediated subtraction (Limes).

@article{HansenHagge2001IdentificationOS,
  title={Identification of sample-specific sequences in mammalian cDNA and genomic DNA by the novel ligation-mediated subtraction (Limes).},
  author={Thomas E. Hansen-Hagge and Uwe Trefzer and A S zu Reventlow and Keld Kaltoft and Wolfram Sterry},
  journal={Nucleic acids research},
  year={2001},
  volume={29 4},
  pages={E20}
}
The representational difference analysis (RDA) and other subtraction techniques are used to enrich sample-specific sequences by elimination of ubiquitous sequences existing in both the sample of interest (tester) and the subtraction partner (driver). While applying the RDA to genomic DNA of cutaneous lymphoma cells in order to identify tumor relevant alterations, we predominantly isolated repetitive sequences and artificial repeat-mediated fusion products of otherwise independent PCR fragments… CONTINUE READING

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