Identification of residues 99, 220, and 221 of human cytochrome P450 2C19 as key determinants of omeprazole activity.

@article{Ibeanu1996IdentificationOR,
  title={Identification of residues 99, 220, and 221 of human cytochrome P450 2C19 as key determinants of omeprazole activity.},
  author={Gordon C. Ibeanu and Burhan I. Ghanayem and Patricia Linko and L Li and Larry G Pederson and Joyce A. Goldstein},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 21},
  pages={12496-501}
}
Human P450 2C19 is selective for 4'-hydroxylation of S-mephenytoin and 5-hydroxylation of omeprazole, while the structurally homologous P450 2C9 has low activity toward these substrates. To identify the critical amino acids that determine the specificity of human amino acids that determine the specificity of human P450 2C19, we constructed chimeras of p450 2C9 replacing various proposed substrate binding sites (SRS) with those of P450 2C19 and then replaced individual residues of P450 2C19 and… CONTINUE READING

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