Identification of reference genes for circulating microRNA analysis in colorectal cancer

@inproceedings{Niu2016IdentificationOR,
  title={Identification of reference genes for circulating microRNA analysis in colorectal cancer},
  author={Yanqin Niu and Yike Wu and Jinyong Huang and Qing Li and Kang Kang and Junle Qu and FuRong Li and Deming Gou},
  booktitle={Scientific reports},
  year={2016}
}
Quantitative real-time PCR (qPCR) is the most frequently used method for measuring expression levels of microRNAs (miRNAs), which is based on normalization to endogenous references. Although circulating miRNAs have been regarded as potential non-invasive biomarker of disease, no study has been performed so far on reference miRNAs for normalization in colorectal cancer. In this study we tried to identify optimal reference miRNAs for qPCR analysis across colorectal cancer patients and healthy… CONTINUE READING
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