Identification of proteins that interact with BRCA1 by Far‐Western library screening

  title={Identification of proteins that interact with BRCA1 by Far‐Western library screening},
  author={Ronit I. Yarden and Lawrence C. Brody},
  journal={Journal of Cellular Biochemistry},
Protein–protein interactions control numerous biological processes. In the case of a protein with no known function, identification of interacting proteins may lend insight into its cellular function. Protein–protein interactions are often detected by yeast two‐hybrid screening which is based on a transcriptional read‐out. One limitation of this technique is that transcription factors, when used as bait, frequently impair the effectiveness of this screen because they give rise to high levels of… 
A homologue of the breast cancer‐associated gene BARD1 is involved in DNA repair in plants
It is demonstrated that in Arabidopsis BARD1 plays a prominent role in the regulation of homologous DNA repair in somatic cells and to a similar extent sensitive to mitomycin C, indicating an epistatic interaction in DNA crosslink repair.
Identification of prion protein binding proteins by combined use of far‐Western immunoblotting, two dimensional gel electrophoresis and mass spectrometry
The successful combination of 2‐DE with far‐Western immunoblotting and MALDI‐TOF MS for identification of new cellular binding partners of a known protein is shown, providing the first evidence of a molecule indicating a mechanism for the predicted involvement of PrPC in nucleic acid metabolisms.
Genetic instability and mammary tumor formation in mice carrying mammary-specific disruption of Chk1 and p53
Data show that Chk1 is critical for maintaining genome integrity and serves as a double-edged sword for cancer: although its inhibition kills cancer cells, it also triggers tumorigenesis when favorable mutations are accumulated for cell growth.
The chromatin remodeling protein BRG1 modulates BRCA1 response to UV irradiation by regulating ATR/ATM activation
It is shown that BRCA1 rapidly binds to UV damage sites when cells are undergoing DNA synthesis, and it is proposed that BRG1 modulates B RCA1 response to UV irradiation by regulating ATM/ATR activation.
Complexes moleculaires brca1/acc alpha, applications diagnostiques et therapeutiques
Cette invention concerne un complexe moleculaire comprenant: un premier polypeptide comprenant la sequence d'acides amines 1640 a 1863 de la proteine BRCA1 humaine ou une meme sequence d'acides


Characterization of a carboxy-terminal BRCA1 interacting protein
Using the yeast two-hybrid and in vitro biochemical assays, it is shown that a protein, CtIP, interacts specifically with the carboxy-terminal segment of human BRCA1 from residues 1602–1863.
BRCA1 interacts with components of the histone deacetylase complex.
  • R. Yarden, L. Brody
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1999
It is demonstrated that BRCA1 interacts with components of the histone deacetylase complex, and therefore may explain the involvement of BRC a1 in multiple processes such as transcription, DNA repair, and recombination.
The C-terminal (BRCT) Domains of BRCA1 Interact in Vivo with CtIP, a Protein Implicated in the CtBP Pathway of Transcriptional Repression*
The results show that the BRCT domains interact in vivo with CtIP, a protein originally identified on the basis of its association with the CtBP transcriptional co-repressor, suggesting that BRCA1 regulates gene expression, at least in part, by modulating CtBP-mediated transcriptional repression.
Evidence for a transcriptional activation function of BRCA1 C-terminal region.
It is shown that the C-terminal region, comprising exons 16-24 (aa 1560-1863) of BRCA1 fused to GAL4 DNA binding domain can activate transcription both in yeast and mammalian cells.
Dual Retinoblastoma-binding Proteins with Properties Related to a Negative Regulator of Ras in Yeast (*)
  • Y. Qian, E. Lee
  • Medicine, Biology
    The Journal of Biological Chemistry
  • 1995
Results suggest that RbAp48 and Rb Ap46 may have shared as well as unique functions in the regulation of cell proliferation and differentiation.
A superfamily of conserved domains in DNA damage‐ responsive cell cycle checkpoint proteins
A large super‐ family of domains that occur predominantly in proteins involved in cell cycle checkpoint functions responsive to DNA damage is revealed, likely to perform critical, yet uncharacterized, functions in the cell cycle control of organisms from bacteria to humans.
Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product
It is reported here that these RBP genes exist in separate loci and produce discrete messenger RNAs and indicate that the cellular RBP-1,RBP-2 and viral E7 proteins interact with pRb through similar domains.
Binding of CtIP to the BRCT Repeats of BRCA1 Involved in the Transcription Regulation of p21 Is Disrupted Upon DNA Damage*
It is suggested that the binding of the BRCT repeats of BRCA1 to CtIP/CtBP is critical in mediating transcriptional regulation of p21 in response to DNA damage.
BRCA1 physically associates with p53 and stimulates its transcriptional activity
BRCA1 increases p53-dependent transcription from the p21WAF1/CIP1 and bax promoters and interacts with p53 proteins both in vitro and in vivo, indicating that BRCA 1 and p53 may coordinately regulate gene expression in their role as tumor suppressors.
Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties
It is found that RBAP-1 copurifies with E2F, interacts specifically with the adenovirus E4 ORF 6/7 protein, binds specifically and directly to a known E2f DNA recognition sequence, and contains a functional tranasactivation domain.