Identification of proteins induced by polycyclic aromatic hydrocarbon in Mycobacterium vanbaalenii PYR‐1 using two‐dimensional polyacrylamide gel electrophoresis and de novo sequencing methods

@article{Kim2004IdentificationOP,
  title={Identification of proteins induced by polycyclic aromatic hydrocarbon in Mycobacterium vanbaalenii PYR‐1 using two‐dimensional polyacrylamide gel electrophoresis and de novo sequencing methods},
  author={Seong-Jae Kim and Richard C. Jones and C. Cha and O. Kweon and R. Edmondson and C. Cerniglia},
  journal={PROTEOMICS},
  year={2004},
  volume={4}
}
Protein profiles of Mycobacterium vanbaalenii PYR‐1 grown in the presence of high‐molecular‐weight polycyclic aromatic hydrocarbons (HMW PAHs) were examined by two‐dimensional gel electrophoresis (2‐DE). Cultures of M. vanbaalenii PYR‐1 were incubated with pyrene, pyrene‐4,5‐quinone (PQ), phenanthrene, anthracene, and fluoranthene. Soluble cellular protein fractions were analyzed and compared, using immobilized pH gradient (IPG) strips. More than 1000 gel‐separated proteins were detected using… Expand
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TLDR
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