Identification of protein interactions by far Western analysis.

@article{Edmondson2001IdentificationOP,
  title={Identification of protein interactions by far Western analysis.},
  author={Diane G. Edmondson and Sharon Y. Roth},
  journal={Current protocols in molecular biology},
  year={2001},
  volume={Chapter 20},
  pages={
          Unit 20.6
        }
}
Far western blotting is a method of identifying protein-protein interactions. One protein of interest is immobilized on a solid support membrane, then probed with a non-antibody protein. Far western blots can be used to identify specific interacting proteins in a complex mixture of proteins. They are particularly useful for examining interactions between proteins that are difficult to analyze by other methods due to solubility problems or because they are difficult to express in cells. This… CONTINUE READING

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