Identification of protein carbonylation sites by two-dimensional liquid chromatography in combination with MALDI- and ESI-MS.

@article{Bollineni2011IdentificationOP,
  title={Identification of protein carbonylation sites by two-dimensional liquid chromatography in combination with MALDI- and ESI-MS.},
  author={Ravi Ch Bollineni and Ralf Hoffmann and Maria Fedorova},
  journal={Journal of proteomics},
  year={2011},
  volume={74 11},
  pages={2338-50}
}
Oxidative stress is defined as excessive production of reactive oxygen species (ROS) overwhelming the cellular antioxidant defense systems and thereby damaging most constituents of cells including proteins. Reactive carbonyls, i.e. aldehydes, ketones and lactams, are a major class of irreversible oxidative protein modifications that are widely used as biomarkers of oxidative stress, aging and age-related diseases. Whereas carbonylated proteins can be studied by western blotting and ELISA, their… CONTINUE READING
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