Identification of protein-binding DNA sequences in an auxin-regulated gene of soybean

  title={Identification of protein-binding DNA sequences in an auxin-regulated gene of soybean},
  author={Ronald T. Nagao and Virginia H. Goekjian and Jong Hong and Joe L. Key},
  journal={Plant Molecular Biology},
The promoter region of a soybean auxin-responsive gene, GmAux28, was analyzed to identify protein-binding DNA sequences that may be involved in regulation of expression. Using DNase I footprinting and gel mobility shift assays, multiple regions of interaction, including eight major protein-binding sites, were observed in the GmAux28 gene. Two sequence motifs, TGACGACA and TCCACGTGTC, related to as-1/Hex and G-box elements, respectively, found in several plant promoters, were identified. Four… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 30 extracted citations


Publications referenced by this paper.
Showing 1-10 of 59 references

Characterization of a nuclear protein from cauliflower which binds a sequence which is sufficient to confer shoot apical meristem specific expres sion to a minimal promoter

ME Williams, Chua N-H
J Cell Biochem 15A: 109 • 1991
View 1 Excerpt

Similar Papers

Loading similar papers…