Identification of prohibitin as a Chikungunya virus receptor protein

@article{Wintachai2012IdentificationOP,
  title={Identification of prohibitin as a Chikungunya virus receptor protein},
  author={Phitchayapak Wintachai and Nitwara Wikan and Atichat Kuadkitkan and Thitigun Jaimipuk and Sukathida Ubol and Rojjanaporn Pulmanausahakul and Prasert Auewarakul and Watchara Kasinrerk and Wenlian Weng and Mingkwan Panyasrivanit and Atchara Paemanee and Suthathip Kittisenachai and Sittiruk Roytrakul and Duncan R. Smith},
  journal={Journal of Medical Virology},
  year={2012},
  volume={84}
}
Chikungunya virus (CHIKV) has recently re‐emerged causing millions of infections in countries around the Indian Ocean. While CHIKV has a broad host cell range and productively infects a number of different cell types, macrophages have been identified as a potential viral reservoir serving to increase the duration of symptoms. To date no CHIKV interacting protein has been characterized and this study sought to identify CHIKV binding proteins expressed on target cell membranes. Two‐dimensional… 
The CD147 Protein Complex Is Involved in Entry of Chikungunya Virus and Related Alphaviruses in Human Cells
TLDR
An affinity purification mass spectrometry coupled approach was designed that allowed the identification of factors that facilitate entry of CHIKV in human cells and showed the involvement of the CD147 protein complex in the replication cycle of related alphaviruses.
Chikungunya Virus Strains from Each Genetic Clade Bind Sulfated Glycosaminoglycans as Attachment Factors
TLDR
GAGs are the preferred glycan bound by CHIKV, enhance the understanding of the specific GAG moieties required forCHIKV binding, define strain differences in GAG engagement, and provide further evidence for a critical function of GAGs in CHikV cell attachment and infection.
Identification of chikungunya virus interacting proteins in mammalian cells
TLDR
Results suggest that chikungunya virus exploits the housekeeping molecules such as actin, HSP70 and STAT-2 to establish infection in the mammalian cells.
Assessment of flavaglines as potential chikungunya virus entry inhibitors
TLDR
All three compounds inhibited infection and reduced virus production when cells were treated before infection but not when added after infection, suggesting that further investigation of prohibitin ligands as potential Chikungunya virus entry inhibitors is warranted.
Global protein profiling studies of chikungunya virus infection identify different proteins but common biological processes
TLDR
While a broad consensus is emerging on which biological processes are altered during CHIKV infection, this consensus is poorly supported in terms of consistent identification of any key proteins mediating those biological processes.
Chikungunya Virus Interacts with Heat Shock Cognate 70 Protein to Facilitate Its Entry into Mosquito Cell Line
TLDR
HSC 70 serves as a putative Chikungunya virus (CHIKV) receptor/s on C6/36 cells that facilitate viral entry and co-IP experiments confirmed the interaction between HSC 70 and CHIKV envelope.
Early Events in Chikungunya Virus Infection—From Virus Cell Binding to Membrane Fusion
TLDR
The aim of the review is to give the reader a state-of-the-art overview on Chikungunya virus cell entry and to provide an outlook on potential new avenues in CHIKV research.
Chikungunya and Its Interaction With the Host Cell
TLDR
This review summarises the CHIKV genome organization, replication, epidemiology, clinical manifestations, and immunopathogenesis with particular emphasis on host-pathogen interactions and concludes that the virus has emerged as a true global pathogen.
The Phosphatidylserine Receptor TIM-1 Enhances Authentic Chikungunya Virus Cell Entry
TLDR
Findings indicate that, unlike Axl, TIM-1 readily promotes the productive entry of authentic CHIKV into target cells, and this work shows that point mutations in the phosphatidylserine binding site of TIM- 1 lead to reduced cell binding, entry, and infection of CHikV.
Host–pathogen interactome analysis of Chikungunya virus envelope proteins E1 and E2
TLDR
The identified interactions shed light on the possible cellular machinery that CHIKV might be employing during viral entry, trafficking, and evasion of immune system.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 76 REFERENCES
Proteomic Analysis of Chikungunya Virus Infected Microgial Cells
TLDR
Analysis of the protein profile in response to infection did not support a global inhibition of either normal or IRES-mediated translation, but was consistent with the targeting of specific cellular pathways including those regulating innate antiviral mechanisms.
Characterization of Reemerging Chikungunya Virus
TLDR
The replication characteristics of recent clinical CHIKV strains are described, indicating that viral entry occurs through pH-dependent endocytosis and general insight is provided into the interaction between chikungunya virus and its mammalian host.
Endocytosis of Chikungunya Virus into Mammalian Cells: Role of Clathrin and Early Endosomal Compartments
TLDR
It is found that CHIKV infection of HEK293T mammalian cells is independent of clathrin heavy chain and- dependent of functional Eps15, and requires integrity of Rab5-, but not Rab7-positive endosomal compartment.
Chikungunya Virus Infection of Cell Lines: Analysis of the East, Central and South African Lineage
TLDR
The results suggest that the increased infectivity seen in insect cells derives from an evolution of the CHIKV genome not solely associated with the E1:226 substitution, as well as generating a comprehensive virus production profile for one of the newer isolates.
Glycoprotein organization of Chikungunya virus particles revealed by X-ray crystallography
TLDR
The crystal structures of the precursor p62–E1 heterodimer and of the mature E3–E2– E1 glycoprotein complexes are reported and provide new insight into the acid-triggered conformational change on the virus particle and its inbuilt inhibition mechanism in the immature complex.
Active Infection of Human Blood Monocytes by Chikungunya Virus Triggers an Innate Immune Response
TLDR
It is demonstrated for the first time that CHIKV Ags could be detected in vivo in the monocytes of acutely infected patients, and monocytes could be infected and virus growth could be sustained, and a better understanding on the basic mechanisms of infection and early antiviral immune responses is provided.
Chikungunya disease in nonhuman primates involves long-term viral persistence in macrophages.
TLDR
In the macaques, long-term CHIKV infection was observed in joints, muscles, lymphoid organs, and liver, which could explain the long-lasting CHikV disease symptoms observed in humans.
...
1
2
3
4
5
...