Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis.

Abstract

Difference in two-dimensional (2-D) gel electrophoresis (DIGE) is a novel method for analyzing up to three samples in one 2-D gel and using the information gained to study post-translational modifications of proteins. We describe the use of DIGE to isolate and characterize those proteins that undergo processing in spermatozoa as they transit the epididymal… (More)

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Cite this paper

@article{Baker2005IdentificationOP, title={Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis.}, author={Mark A. Baker and Roxanne Witherdin and Louise Hetherington and Kelly Cunningham-Smith and Rachel J. Aitken}, journal={Proteomics}, year={2005}, volume={5 4}, pages={1003-12} }