Identification of plant microRNAs using expressed sequence tag analysis.

@article{Frazier2011IdentificationOP,
  title={Identification of plant microRNAs using expressed sequence tag analysis.},
  author={Taylor Price Frazier and Baohong Zhang},
  journal={Methods in molecular biology},
  year={2011},
  volume={678},
  pages={
          13-25
        }
}
microRNAs (miRNAs) are a new class of small endogenous noncoding regulatory RNAs, which play an important function in plant growth, development, phase change, and response to environmental stress. Identifying miRNAs is the first step for investigating miRNA-mediated gene regulation and miRNA function. In this chapter, we describe a comprehensive comparative genomics-based expressed sequence tag (EST) analysis for identifying miRNAs from a wide range of plant species. EST analysis is based on… 
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