Identification of phenylthiocarbamyl amino acids for compositional analysis by thermospray liquid chromatography/mass spectrometry.


The chromatographic separation of amino acids for compositional analysis of peptides and proteins is commonly performed by reverse-phase high-performance liquid chromatography of amino acid residues that have been derivatized with phenylisothiocyanate. The present report describes an extension of this method, which employs thermospray liquid chromatography… (More)


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