Identification of phenolic compounds in aqueous and ethanolic rooibos extracts (Aspalathus linearis) by HPLC-ESI-MS (TOF/IT)

  title={Identification of phenolic compounds in aqueous and ethanolic rooibos extracts (Aspalathus linearis) by HPLC-ESI-MS (TOF/IT)},
  author={Ihsan Iswaldi and David Arr{\'a}ez-Rom{\'a}n and Inmaculada C. Rodr{\'i}guez-Medina and Ra{\'u}l Beltr{\'a}n-Deb{\'o}n and Jorge Joven and Antonio Segura‐Carretero and Alberto Fernandez-Guti{\'e}rrez},
  journal={Analytical and Bioanalytical Chemistry},
Rooibos (Aspalathus linearis) is a rich source of polyphenols and used to make a mild-tasting tea containing no caffeine, is low in tannins compared to green or black teas, and has antioxidant and antimutagenic/antitumoral properties. In vivo results show that rooibos has beneficial effects upon the lipid profile by decreasing serum triglycerides and cholesterol. In this sense, we have developed a simple and rapid method to separate and characterize simultaneously the polyphenolic compounds in… 
Characterization and identification of the major flavonoids in Phyllostachys edulis leaf extract by UPLC–QTOF–MS/MS
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A Prospective of Multiple Biopharmaceutical Activities of Procyanidins‐Rich Uapaca togoensis Pax Extracts: HPLC‐ESI‐TOF‐MS Coupled with Bioinformatics Analysis
Uapaca togoensis might be valuable as potential source of natural agents for therapeutic application as the tested extracts have shown wide-spectrum antibacterial properties and these effects have shown to be more effective against Aspergillus ochraceus and Penicillium funiculosum.
The characterisation and delivery of flavonoids and other minor components in traditional food ingredients.
The primary aim of this research was to determine whether herbal infusions, yerba mate (Ilex paraguariensis A. St.-Hilaire) and rooibos (Aspalathus linearis (Burm. F.) R. Dahlgren), could make a
Analysis of Phenolic Compounds in Rooibos Tea (Aspalathus linearis) with a Comparison of Flavonoid-Based Compounds in Natural Populations of Plants from Different Regions.
The collisional cross-section data obtained from ion mobility-mass spectrometry is reported for the flavonoids in rooibos for the first time, facilitating the identification of several new compounds as well as grouping of the wild tea ecotypes according to their chemical composition.
Approach to the study of flavone di-C-glycosides by high performance liquid chromatography-tandem ion trap mass spectrometry and its application to characterization of flavonoid composition in Viola yedoensis.
It is reported that the MS(2) and MS(3) structure-diagnostic fragments about the glycosylation types and positions are highly dependent on the configuration of the sugars at C-6 and C-8.
Impact of Cold versus Hot Brewing on the Phenolic Profile and Antioxidant Capacity of Rooibos (Aspalathus linearis) Herbal Tea
The results contribute to a better understanding of the impact of the preparation method on the potential health benefits of rooibos tea, especially for flavonoids and phenolic acids.


Characterization of phenolic compounds in rooibos tea.
Prepative high-performance liquid chromatography was applied to obtain pure flavonoids and the purity and identity of isolated compounds was confirmed by different NMR experiments, HPLC-diode array detector (DAD), or gas chromatography-mass spectrometry (GC-MS) analysis.
Quantitative characterization of flavonoid compounds in Rooibos tea (Aspalathus linearis) by LC-UV/DAD.
An HPLC method using a C(18) reversed phase column was developed for the assay of 10 flavonoids in aqueous and methanolic infusions and showed evidence of the presence of other compounds such as nothofagin, diHydroisoorientin, and dihydroorientin.
Qualitative screening of phenolic compounds in olive leaf extracts by hyphenated liquid chromatography and preliminary evaluation of cytotoxic activity against human breast cancer cells
Olive leaf extracts exhibited dose-dependent inhibitory effects on the metabolic status (cell viability) of three breast cancer models in vitro, and warrant further investigation at the structure–function molecular level to definitely establish the anticancer value of these phytochemicals.
Liquid chromatography-tandem mass spectrometric method for determination of the anti-inflammatory compound vicenin-2 in the leaves of L. ericoides Mart.
A rapid and sensitive method for determination of the anti-inflammatory compound vicenin-2 in L. ericoides Mart.
Comparison of the Antioxidant Activity of Aspalathin with That of Other Plant Phenols of Rooibos Tea (Aspalathus linearis), α-Tocopherol, BHT, and BHA
The antioxidant activity of aspalathin, the major flavonoid of unfermented rooibos tea, was compared with that of other polyphenols present in rooibos tea, α-tocopherol, BHT, and BHA using the
Continuous administration of polyphenols from aqueous rooibos (Aspalathus linearis) extract ameliorates dietary-induced metabolic disturbances in hyperlipidemic mice.