Identification of pexophagy genes by restriction enzyme-mediated integration.

  title={Identification of pexophagy genes by restriction enzyme-mediated integration.},
  author={Laura Aaron Schroder and Benjamin S. Glick and W Augustine Dunn},
  journal={Methods in molecular biology},
Pichia pastoris has proven to be a valuable model for examining the molecular events of the selective degradation of peroxisomes by a process called pexophagy. We have developed a protocol to rapidly identify genes essential for glucose-induced pexophagy. This method utilizes the random integration of a Zeocin resistance cassette vector into the genomic DNA thereby disrupting gene expression. Transformed yeast are selected by growth on Zeocin and pexophagy mutants identified by their inability… CONTINUE READING

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