Identification of peptidases in highly pathogenic vs. weakly pathogenic Naegleria fowleri amebae.

@article{Vyas2015IdentificationOP,
  title={Identification of peptidases in highly pathogenic vs. weakly pathogenic Naegleria fowleri amebae.},
  author={Ishan K Vyas and Melissa J Jamerson and Guy A. Cabral and Francine Marciano-Cabral},
  journal={The Journal of eukaryotic microbiology},
  year={2015},
  volume={62 1},
  pages={
          51-9
        }
}
Naegleria fowleri, a free-living ameba, is the causative agent of Primary Amebic Meningoencephalitis. Highly pathogenic mouse-passaged amebae (Mp) and weakly pathogenic axenically grown (Ax) N. fowleri were examined for peptidase activity. Zymography and azocasein peptidase activity assays demonstrated that Mp and Ax N. fowleri exhibited a similar peptidase pattern. Prominent for whole cell lysates, membranes and conditioned medium (CM) from Mp and Ax amebae was the presence of an activity band… CONTINUE READING

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