Identification of pathogenic yeast species by polymerase chain reaction amplification of the RPS0 gene intron fragment.

@article{Martnez2010IdentificationOP,
  title={Identification of pathogenic yeast species by polymerase chain reaction amplification of the RPS0 gene intron fragment.},
  author={J M Garc{\'i}a Mart{\'i}nez and Eulogio Valent{\'i}n G{\'o}mez and Javier Pem{\'a}n and Emilia Cant{\'o}n and M. G. Garcia and Lucas del Castillo Agudo},
  journal={Journal of applied microbiology},
  year={2010},
  volume={108 6},
  pages={1917-27}
}
AIMS This work focuses on the development of a method for the identification of pathogenic yeast. With this aim, we target the nucleotide sequence of the RPS0 gene of pathogenic yeast species with specific PCR primers. PCR analysis was performed with both the genomic DNA, whole cells of clinical isolates of Candida species and clinical samples. METHODS AND RESULTS A single pairs of primers, deduced from the nucleotide sequence of the RPS0 gene from pathogenic yeast, were used in PCR analysis… CONTINUE READING
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