Identification of p53 as a sequence-specific DNA-binding protein

  title={Identification of p53 as a sequence-specific DNA-binding protein},
  author={S. Kern and K. Kinzler and A. Bruskin and D. Jarosz and P. Friedman and C. Prives and B. Vogelstein},
  pages={1708 - 1711}
  • S. Kern, K. Kinzler, +4 authors B. Vogelstein
  • Published 1991
  • Biology, Medicine
  • Science
  • The tumor-suppressor gene p53 is altered by missense mutation in numerous human malignancies. However, the biochemical properties of p53 and the effect of mutation on these properties are unclear. A human DNA sequence was identified that binds specifically to wild-type human p53 protein in vitro. As few as 33 base pairs were sufficient to confer specific binding. Certain guanines within this 33-base pair region were critical, as methylation of these guanines or their substitution with thymine… CONTINUE READING
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