Identification of p53 as a sequence-specific DNA-binding protein

@article{Kern1991IdentificationOP,
  title={Identification of p53 as a sequence-specific DNA-binding protein},
  author={S. Kern and K. Kinzler and A. Bruskin and D. Jarosz and P. Friedman and C. Prives and B. Vogelstein},
  journal={Science},
  year={1991},
  volume={252},
  pages={1708 - 1711}
}
  • S. Kern, K. Kinzler, +4 authors B. Vogelstein
  • Published 1991
  • Biology, Medicine
  • Science
    • The tumor-suppressor gene p53 is altered by missense mutation in numerous human malignancies. However, the biochemical properties of p53 and the effect of mutation on these properties are unclear. A human DNA sequence was identified that binds specifically to wild-type human p53 protein in vitro. As few as 33 base pairs were sufficient to confer specific binding. Certain guanines within this 33-base pair region were critical, as methylation of these guanines or their substitution with thymine… CONTINUE READING
    View on AAAS
    ncbi.nlm.nih.gov
    997 Citations
    Highly Influencial Citations
    29
    Background Citations
    276
    Methods Citations
    38
    Results Citations
    4

    Topics from this paper.

    Explore Further: Topics Discussed in This Paper

    997 Citations
    Citation Type

    Citation Type

    Analysis of a protein-binding domain of p53.
    • 55
    • PDF
    Sequence-specific interaction of a conformational domain of p53 with DNA.
    • 29
    • PDF
    Regulation of the specific DNA binding function of p53
    • 891
    Identification of a minimal transforming domain of p53: negative dominance through abrogation of sequence-specific DNA binding.
    • 360
    • PDF
    Oncogenic forms of p53 inhibit p53-regulated gene expression
    • 507
    Definition of a consensus binding site for p53
    • 1,916
    Specific recognition of a transcriptional element within the human h-ras protooncogene by the p53 tumor-suppressor.
    • 9
    • PDF
    Identification of partial loss of function p53 gene mutations utilizing a yeast-based functional assay.
    • 3
    • PDF
    The functions of the p53 gene and gene product
    Wild-type but not mutant p53 immunopurified proteins bind to sequences adjacent to the SV40 origin of replication
    • 316

    References

    SHOWING 1-10 OF 34 REFERENCES
    Mutant p53 proteins bind DNA abnormally in vitro.
    • 144
    Presence of a potent transcription activating sequence in the p53 protein.
    • 713
    DNA binding properties of murine p53.
    • 78
    Identification and characterization of a p53 gene mutation in a human osteosarcoma cell line.
    • 100
    p53: a frequent target for genetic abnormalities in lung cancer.
    • 1,178
    Transcriptional activation by wild-type but not transforming mutants of the p53 anti-oncogene.
    • 557
    p53 functions as a cell cycle control protein in osteosarcomas.
    • 756
    • PDF
    Identification of intronic point mutations as an alternative mechanism for p53 inactivation in lung cancer.
    • 138
    Genetic and immunochemical analysis of mutant p53 in human breast cancer cell lines.
    • 610
    Mutations in the p53 gene occur in diverse human tumour types
    • 2,623
    • PDF