Identification of osteoblast/osteocyte factor 45 (OF45), a bone-specific cDNA encoding an RGD-containing protein that is highly expressed in osteoblasts and osteocytes.

@article{Petersen2000IdentificationOO,
  title={Identification of osteoblast/osteocyte factor 45 (OF45), a bone-specific cDNA encoding an RGD-containing protein that is highly expressed in osteoblasts and osteocytes.},
  author={Donna N. Petersen and George T Tkalcevic and Amy L Mansolf and R Rivera-Gonzalez and Thomas A. Brown},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 46},
  pages={36172-80}
}
We describe the cloning and characterization of a novel bone-specific cDNA predicted to encode an extracellular matrix protein. This cDNA was identified by subtractive hybridization based upon its high expression in bone marrow-derived osteoblasts. By Northern blot analysis, we detected a single 2-kilobase mRNA transcript in bone, whereas no expression was detected in other tissues. Immunohistochemistry revealed that the protein was expressed highly in osteocytes within trabecular and cortical… CONTINUE READING

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