Identification of orthopaedic infections using broad-range polymerase chain reaction and reverse line blot hybridization.

@article{Moojen2007IdentificationOO,
  title={Identification of orthopaedic infections using broad-range polymerase chain reaction and reverse line blot hybridization.},
  author={Dirk Jan F. Moojen and Sanne N. M. Spijkers and Corrie S. Schot and Marc W. Nijhof and H Charles Vogely and Andr{\'e} Fleer and Abraham J. Verbout and Ren{\'e} M. Castelein and Wouter J. A. Dhert and Leo M. Schouls},
  journal={The Journal of bone and joint surgery. American volume},
  year={2007},
  volume={89 6},
  pages={1298-305}
}
BACKGROUND Culture remains the gold standard in the diagnosis of bacterial infection, but molecular biological techniques have yielded promising results. In this study, we validated a combined polymerase chain reaction and reverse line blot hybridization protocol for identifying musculoskeletal infections. METHODS Samples were obtained from seventy-six patients undergoing orthopaedic surgery for various aseptic and septic indications. The diagnosis of infection was based on a review of all… CONTINUE READING

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