Identification of novel metabolites from Aspergillus flavus by high resolution and multiple stage mass spectrometry

@article{Malysheva2014IdentificationON,
  title={Identification of novel metabolites from Aspergillus flavus by high resolution and multiple stage mass spectrometry},
  author={Svetlana V. Malysheva and Natalia Arroyo-Manzanares and Jeffrey W. Cary and Kenneth C. Ehrlich and Julie Vanden Bussche and Lynn Vanhaecke and Deepak Bhatnagar and Jos{\'e} Diana di Mavungu and Sarah de Saeger},
  journal={Food Additives \& Contaminants: Part A},
  year={2014},
  volume={31},
  pages={111 - 120}
}
The filamentous fungus Aspergillus flavus is one of the most important species in the Aspergillus genus and is distributed worldwide as a prevalent aflatoxin-producing food and feed contaminant. A. flavus contains more than 55 gene clusters that are predicted to encode proteins involved in secondary metabolite production. One of these, cluster 27, contains a polyketide synthase (pks27) gene that encodes a protein that is highly homologous to the aflatoxin cluster PKS. Comparative metabolomics… 
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24 Citations

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Use of UHPLC high-resolution Orbitrap mass spectrometry to investigate the genes involved in the production of secondary metabolites in Aspergillus flavus
TLDR
An analytical methodology based on Orbitrap HRMS technology that allows the untargeted determination of fungal metabolites, in support of the study of the function of genes involved in secondary metabolism in fungi is described.
Aspergillus flavus Secondary Metabolites: More than Just Aflatoxins.
TLDR
The genetic, biochemical and bioinformatic methods that are being used to identify previously uncharacterized secondary metabolite gene clusters and their associated metabolites are discussed.
Comparison of Expression of Secondary Metabolite Biosynthesis Cluster Genes in Aspergillus flavus, A. parasiticus, and A. oryzae
TLDR
RNA-seq is used to compare expression of secondary metabolite genes in gene clusters for the closely related fungi A. parasiticus, A. oryzae, and A. flavus S and L sclerotial morphotypes to refine the identification of probable functional gene clusters within these species.
Secondary Metabolite Dereplication and Phylogenetic Analysis Identify Various Emerging Mycotoxins and Reveal the High Intra-Species Diversity in Aspergillus flavus
Aspergillus flavus is one of the most important mycotoxigenic species from the genus Aspergillus, due to its ability to synthesize the potent hepatocarcinogen, aflatoxin B1. Moreover, this fungus is…
Functional characterization of a veA-dependent polyketide synthase gene in Aspergillus flavus necessary for the synthesis of asparasone, a sclerotium-specific pigment.
Aspergillus flavus aswA, a gene homolog of Aspergillus nidulans oefC, regulates sclerotial development and biosynthesis of sclerotium-associated secondary metabolites.
Metabolites Identified during Varied Doses of Aspergillus Species in Zea mays Grains, and Their Correlation with Aflatoxin Levels
TLDR
Investigation of metabolites produced during fungal development on maize and their correlation with aflatoxin levels found several polar metabolites were important for dose classification and several metabolites were interconnected with the TCA cycle.
Aspergillus korhogoensis, a Novel Aflatoxin Producing Species from the Côte d’Ivoire
TLDR
Results confirm the monophyly of A. korhogoensis, and strengthen its position in the A. flavus clade, as the sister taxon of Aspergillus parvisclerotigenus.
The Aspergilli and Their Mycotoxins: Metabolic Interactions With Plants and the Soil Biota
TLDR
The role of mycotoxin producing Aspergillus species and their interactions in the ecosystem are discussed and the complexity of the roles of the main toxic secondary metabolites as well as their fate in natural environments and agriculture is highlighted.
Taxonomy of Aspergillus section Flavi and their production of aflatoxins, ochratoxins and other mycotoxins
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