Identification of novel FLT-3 Asp835 mutations in adult acute myeloid leukaemia.

@article{AbuDuhier2001IdentificationON,
  title={Identification of novel FLT-3 Asp835 mutations in adult acute myeloid leukaemia.},
  author={Faisel M. Abu-Duhier and Anne C Goodeve and Gill A. Wilson and Rory S Care and Ian Peake and John T. Reilly},
  journal={British journal of haematology},
  year={2001},
  volume={113 4},
  pages={983-8}
}
Genomic DNA from 97 cases of adult de novo acute myeloid leukaemia (AML) was screened using polymerase chain reaction (PCR) and conformation-sensitive gel electrophoresis (CSGE) for FLT3 exon 20 mutations. Initial sequencing of four cases, representing the spectrum of CSGE abnormalities, revealed changes affecting codon Asp835 in three cases and also an intron 20 A to G change. In order to identify all possible Asp835 alterations, as well as the frequency of the intronic change nucleotide 2541… CONTINUE READING