Identification of novel Escherichia coli ribosome-associated proteins using isobaric tags and multidimensional protein identification techniques.

@article{Jiang2007IdentificationON,
  title={Identification of novel Escherichia coli ribosome-associated proteins using isobaric tags and multidimensional protein identification techniques.},
  author={Mengxi Jiang and Susan T. McLain Sullivan and Angela K. Walker and John R. Strahler and Philip C. Andrews and J. Maddock},
  journal={Journal of bacteriology},
  year={2007},
  volume={189 9},
  pages={3434-44}
}
Biogenesis of the large ribosomal subunit requires the coordinate assembly of two rRNAs and 33 ribosomal proteins. In vivo, additional ribosome assembly factors, such as helicases, GTPases, pseudouridine synthetases, and methyltransferases, are also critical for ribosome assembly. To identify novel ribosome-associated proteins, we used a proteomic approach (isotope tagging for relative and absolute quantitation) that allows for semiquantitation of proteins from complex protein mixtures… CONTINUE READING

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