Identification of novel ERK2 substrates through use of an engineered kinase and ATP analogs.

  title={Identification of novel ERK2 substrates through use of an engineered kinase and ATP analogs.},
  author={Scott T. Eblen and Niranjana Kumar and Kavita Shah and Michelle J. Henderson and Colin K. W. Watts and Kevan M. Shokat and Michael D Weber},
  journal={The Journal of biological chemistry},
  volume={278 17},
The mitogen-activated protein kinases are key regulators of cellular organization and function. To understand the mechanisms(s) by which these ubiquitous kinases affect specific cellular changes, it is necessary to identify their diverse and numerous substrates in different cell contexts and compartments. As a first step in achieving this goal, we engineered a mutant ERK2 in which a bulky amino acid residue in the ATP binding site (glutamine 103) is changed to glycine, allowing this mutant to… CONTINUE READING


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