Identification of nitration sites on surfactant protein A by tandem electrospray mass spectrometry.

@article{Greis1996IdentificationON,
  title={Identification of nitration sites on surfactant protein A by tandem electrospray mass spectrometry.},
  author={Kenneth D. Greis and Sheng-geng Zhu and Sadis Matalon},
  journal={Archives of biochemistry and biophysics},
  year={1996},
  volume={335 2},
  pages={396-402}
}
Previous studies have shown that exposure of human surfactant protein A (SP-A) to nitrating agents [peroxynitrite (ONOO-); tetranitromethane (TNM; pH 8)] leads to nitrotyrosine formation. However, specific sites of nitration have not been identified. Herein, human SP-A, dissolved in Hepes buffer, was incubated with two boluses each of 0.5 mM ONOO- (pH 7.4… CONTINUE READING