Identification of new genes involved in the virulence of Listeria monocytogenes by signature-tagged transposon mutagenesis.

@article{Autret2001IdentificationON,
  title={Identification of new genes involved in the virulence of Listeria monocytogenes by signature-tagged transposon mutagenesis.},
  author={Nicolas Autret and Iharilalao Dubail and Patrick Trieu-Cuot and Patrick Berche and Alain Charbit},
  journal={Infection and immunity},
  year={2001},
  volume={69 4},
  pages={2054-65}
}
Listeria monocytogenes is a gram-positive, facultative intracellular pathogen that can cause severe food-born infections in humans and animals. We have adapted signature-tagged transposon mutagenesis to L. monocytogenes to identify new genes involved in virulence in the murine model of infection. We used transposon Tn1545 carried on the integrative vector pAT113. Forty-eight tagged transposons were constructed and used to generate banks of L. monocytogenes mutants. Pools of 48 mutants were… CONTINUE READING

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