Identification of mycobacteria by high-performance liquid chromatography.

  title={Identification of mycobacteria by high-performance liquid chromatography.},
  author={W. Ray Butler and Kenneth C. Jost and Jennifer Kilburn},
  journal={Journal of clinical microbiology},
  volume={29 11},
Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a differentiation scheme that was 98% accurate for the species examined. A rapid, cost-effective HPLC method… CONTINUE READING

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